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Image Search Results
Journal: Molecular Therapy. Nucleic Acids
Article Title: Reprogramming of Urine-Derived Renal Epithelial Cells into iPSCs Using srRNA and Consecutive Differentiation into Beating Cardiomyocytes
doi: 10.1016/j.omtn.2019.07.016
Figure Lengend Snippet: In Vitro Differentiation Potential of iPSCs Derived from RECs into the Three Germ Layers: Mesoderm, Endoderm, and Ectoderm After 7 days of trilineage differentiation, cells with changed phenotypes (indicated with arrows) were observed. Flow cytometry analysis confirmed the differentiation of iPSCs (passages 3–7) into all three germ layers by detection of mesodermal (CD31 and SMA), definitive-endodermal (AFP and CXCR4), and neuroectodermal (PAX6 and TUBB3) cells. Compared to controls, differentiated cells showed significantly increased expression of mesodermal, endodermal, and ectodermal markers. Scale bars of phase contrast microscopic pictures represent 100 μm. Results are shown as mean + SD (n = 4). Statistical differences were determined using one-way ANOVA followed by Bonferroni’s multiple comparison test (****p < 0.0001).
Article Snippet: Ectodermal differentiation potential was assessed through the presence of neuroectoderm cells using PE-labeled
Techniques: In Vitro, Derivative Assay, Flow Cytometry, Expressing, Comparison